FastQCFastQC Report
Mon 6 Jun 2016
ERR1378134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140417
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGTAATCTCGTATGCC16410.143894733242314Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1500.038.9986342
GACCGAT1500.038.99692529
CGTATGC1600.036.5612244
CCGATAG1600.036.5612231
ACCGATA1650.035.45174830
AGACCGA1800.034.99723428
GAGACCG1950.032.3051427
TCGTATG1900.031.97256343
TCTCGTA1850.031.62051241
GCGGTTC2050.029.630518
AGCGGTT2150.029.2987297
GAGCGGT2100.028.9427876
CGGTTCA2100.028.925029
CGATAGT2050.028.53558532
CGGAAGA2150.028.274661
CCGAGAC2250.026.9978725
ATCTCGT2200.026.58997740
AAGAGCG2300.026.4260224
AATCTCG2250.025.99908639
AGAGCGG2350.025.8637665
Produced by FastQC (version 0.11.5)