FastQCFastQC Report
Mon 6 Jun 2016
ERR1378133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242119
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTATAATCTCGTATGCC12840.10337173813459097Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC250.002100224836.0242351
CGAGTAT2200.033.74960732
TCTCGTA2200.033.74960741
CGTATGC2250.032.9996244
GACCGAG2250.032.9969629
CTCGTAT2400.030.9371442
ACCGAGT2400.030.9346530
CGAACCA300.00514973629.99723643
AGACCGA2550.029.99723628
TCGTATG2500.029.69726643
GAGACCG2600.028.55391127
CCGAGAC2600.028.55391125
ATCTCGT2550.028.23496840
GAGCGGT2800.028.13946
GATCGGA803.4015102E-928.12127718
CGGTTCA2750.027.81459
CGGAAGA2850.027.6501831
AATCTCG2650.027.16949539
AGATCGG856.491973E-926.46708517
GTATGCC2850.026.0523345
Produced by FastQC (version 0.11.5)