FastQCFastQC Report
Mon 6 Jun 2016
ERR1378132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517266
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA1600.040.77969729
CCGTAGA1700.039.7056831
CTCGTAT1850.038.91871642
CGTAGAT1900.036.7103432
CGTATGC2050.035.12177344
GAGACCG2000.033.7487227
TCTCGTA2200.032.72710441
TCGTATG2200.031.70438443
GATCGGA501.9837807E-631.49672718
ACCGTAG2150.031.39415230
GCGGTTC2150.031.3920828
ATCTCGT2300.031.3041940
AGACCGT2100.031.07024628
GAGCGGT2200.030.688746
CGGTTCA2350.029.6777619
ATGCCGA2450.028.46736522
AGCGGTT2500.026.9971927
AGAGCGG2550.026.4765595
GGTTGCG450.001227008124.99904827
AATGCCG2800.024.90894521
Produced by FastQC (version 0.11.5)