FastQCFastQC Report
Mon 6 Jun 2016
ERR1378131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016392
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATGAATCTCGTATGCC16160.1589937740556793Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATA2000.040.496130
GACCGAT2000.039.37121229
CGTATGC2150.038.7191144
CGATATG2150.038.7191132
CCGATAT2100.038.56961431
TCGTATG2250.036.9964443
CGGTTCA2350.036.379489
TCTCGTA2300.036.1939541
AATCTCG2300.036.1939539
GAGACCG2250.034.9966327
AGACCGA2300.034.2358428
GCGGTTC2500.034.1967128
GAGCGGT2550.033.544346
AGAGCGG2550.033.544345
CTCGTAT2500.033.29843542
AGCGGTT2600.032.881457
ATCTCGT2550.032.64552340
CCGAGAC2500.032.39688525
AAGAGCG2850.031.5930064
CGGAAGA2850.030.0251771
Produced by FastQC (version 0.11.5)