FastQCFastQC Report
Mon 6 Jun 2016
ERR1378130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374513
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG1900.031.97164332
GACCGTA1850.031.61845229
CGTATGC1900.030.78750844
CTCGTAT1950.029.99808342
GCGATTA453.4937264E-529.99699420
GCGCCTA300.0051501329.99699228
GCGGTTC2150.029.299398
AGACCGT2000.029.24706628
AGAGCGG2100.028.9425165
TCTCGTA2050.028.53476341
GAGACCG2050.028.53372627
CCGTATA2150.028.25401131
GAGCGGT2200.027.6269476
ACCGTAT2150.027.20657730
CGGTTCA2450.026.6299829
ATCTCGT2200.026.5892140
AGCGGTT2400.026.2473687
TCGTATG2250.025.99833943
CCGAGAC2400.025.30996125
CGGAAGA2450.024.8150981
Produced by FastQC (version 0.11.5)