FastQCFastQC Report
Mon 6 Jun 2016
ERR1378128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686956
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTACCATCTCGTATGCCGTC23230.3381584846773301Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTACCATCTCGTATGCC15800.23000017468367698Illumina Paired End PCR Primer 2 (96% over 33bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA11210.1631836682407607No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA8440.12286085280571099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA1350.043.32730524
AGCCGAT1300.043.26321415
GCCGATG1300.043.26321416
TAAGCCG1300.043.26321413
CGGTATT1150.041.08124524
CCGATGA1400.040.1729817
TTCGGTA1200.039.3695222
TCGGTAT1200.039.3695223
TGACGTC1550.039.188121
TGCCGTC2150.038.7324645
AGGCGGT1300.038.0716334
GACGTCA1550.037.73668722
CGATGAC1550.037.73668718
AAGCCGA1500.037.4947814
GGCGGTC1350.036.66156835
ATGACGT1600.036.55741520
ATGCCGT2400.035.6200444
GCGGTCT1400.035.35222636
AATTCGG1400.035.35222620
TATGCCG2400.034.6826743
Produced by FastQC (version 0.11.5)