FastQCFastQC Report
Mon 6 Jun 2016
ERR1378127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692600
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA16970.24501876985272886No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCCTGATCTCGTATGCCGTC16940.24458561940514006Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCCTGATCTCGTATGCC11750.16965059197227836Illumina Paired End PCR Primer 2 (96% over 33bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA10990.15867744730002886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTC1250.041.3936935
GACGTCA2600.039.80162422
TGACGTC2600.039.80162421
AGGCGGT1300.039.80162434
CGTCATA2550.039.69983324
CCGATGA2600.038.93637517
ACGTCAT2600.038.93637523
TAAGCCG2600.038.93637513
TGCCGTC2050.038.41432645
TATGCCG2000.038.2441743
GCCGATG2650.038.20172516
GCGCTCC301.13612055E-437.518668
ATGACGT2700.037.49428620
CGATGAC2700.037.49428618
TCGGTAT1450.037.23570323
AGCCGAT2750.036.8125715
GATGACG2800.036.155219
GCCGTCA250.00210810835.99451444
CGGTATT1500.035.9945124
GCGGTCT1450.035.68421636
Produced by FastQC (version 0.11.5)