FastQCFastQC Report
Mon 6 Jun 2016
ERR1378126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472600
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA12850.27190012695725774No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATATAATCTCGTATGCCGTC11710.24777824798984344Illumina Paired End PCR Primer 2 (96% over 30bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA8980.1900126957257723No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATATAATCTCGTATGCC8470.1792213288192975Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGA1200.041.24534217
GCCGATG1200.041.24534216
AGATTCG501.0750227E-940.5168610
AGCCGAT1250.039.59552815
ATTCGGT1550.037.7376821
CGGGGAA1550.037.7376815
TTCGGTA1550.037.7376822
GCGGTCT1500.037.4957736
GGCGGTC1500.037.4957735
TCGGTAT1650.036.8140323
ACGTCAT1350.036.6625323
TAAGCCG1350.036.6625313
TATGCCG1050.036.4244643
CGGTATT1650.035.45054624
GATGACG1400.035.35315319
CGTCATA1400.035.35315324
AGGCGGT1600.035.1522834
TGCCGTC1100.034.7798445
AATTCGG1750.034.71036520
GAATTCG1700.034.40788319
Produced by FastQC (version 0.11.5)