FastQCFastQC Report
Mon 6 Jun 2016
ERR1378122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717968
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA15980.22257259376462463No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTACGATCTCGTATGCCGTC14520.20223742562342611Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTACGATCTCGTATGCC13890.19346266128852538Illumina Paired End PCR Primer 2 (96% over 33bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA11230.15641365631894458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCAT1800.042.49664323
ATGACGT1850.041.34808720
TAAGCCG1850.041.3452113
TGACGTC1950.039.22767321
CGATGAC1950.039.22767318
GGCGGTC1700.038.37932635
AATTCGG1700.038.37932620
TCGGTAT1700.038.37932623
CCGATGA2000.038.2469817
GATGACG2000.038.2469819
CGTCATA2000.038.2469824
AGCCGAT2000.038.24431615
GCCGATG2050.037.3141316
GACGTCA2100.036.42569722
AAGCCGA2150.035.5761114
GAATTCG1900.035.52351419
ATGCCGT1650.035.44927644
GCGGTCT1850.035.26748736
ATTCGGT1850.035.26748721
TTCGGTA1850.035.26748722
Produced by FastQC (version 0.11.5)