FastQCFastQC Report
Mon 6 Jun 2016
ERR1378121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009008
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA17780.17621267621267622No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTGAATCTCGTATGCCGTC16790.16640105925820212Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTGAATCTCGTATGCC15820.1567876567876568Illumina Paired End PCR Primer 2 (97% over 34bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA12000.1189286903572618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA2000.040.4945124
GCGGTCT2250.039.99458336
GGCGGTC2250.039.99458335
TGACGTC2050.039.50684421
GCCGATG2050.039.50684416
CGATGAC2050.039.50684418
TCGGTAT2300.039.12513423
GACGTCA2100.038.56620422
TAAGCCG2200.037.83578513
CCGATGA2150.037.66931517
ACGTCAT2150.037.66931523
CGGTATT2400.037.4949224
AGCCGAT2250.036.9949915
CGGTCTC2450.036.7297237
AAGCCGA2250.035.99512514
TTCGGTA2550.035.28933722
TATGCCG2050.035.11719543
ATGCCGT2150.034.53020544
GATGACG2350.034.46341719
AGGCGGT2700.034.1620434
Produced by FastQC (version 0.11.5)