FastQCFastQC Report
Mon 6 Jun 2016
ERR1378120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784733
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGGCCATCTCGTATGCCGTC23960.3053267799366154Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGGCCATCTCGTATGCC19470.24810986666802592Illumina Paired End PCR Primer 2 (97% over 35bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA16440.20949800760258586No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA11400.14527234103828945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAT1600.043.5907923
GGCGGTC1600.042.18463535
GCCGATG2050.041.70448316
GATGACG2050.041.70448319
CGATGAC2050.041.70448318
TTCGGTA1700.041.02662322
ATGACGT2100.040.71151720
TAAGCCG2100.040.71151713
CCGATGA2150.039.7647417
GACGTCA2150.039.7647422
AGGCGGT1700.039.70318234
ACGTCAT2200.038.86099623
GCGGTCT1750.038.56880636
AGCCGAT2250.037.99741415
TGACGTC2250.037.99741421
TGCCGTC2550.037.95552445
TTCCTCG1550.037.75139610
AATTCGG1800.037.4974520
CGTCATA2300.037.17138724
CGGTATT1950.035.766824
Produced by FastQC (version 0.11.5)