FastQCFastQC Report
Mon 6 Jun 2016
ERR1378119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726176
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGGTTTATCTCGTATGCCGTC16890.23258824307055037Illumina Paired End PCR Primer 2 (96% over 31bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA12320.16965584100824044No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGGTTTATCTCGTATGCC11960.16469836513462302Illumina Paired End PCR Primer 2 (97% over 34bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA8990.12379918917727933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTC1450.040.3420535
GACGTCA1550.039.19085322
TGACGTC1550.039.19085321
GCCGATG1400.038.56877516
CGATGAC1400.038.56877518
TTCGGTA1600.037.96613722
ATGACGT1500.037.49741720
AGCCGAT1450.037.23881515
CCGATGA1450.037.23881517
GCGGTCT1600.036.55998236
GAATTCG1600.036.55998219
TCGGTAT1600.036.55998223
ATGCCGT2800.036.15822644
TGCCGTC2750.036.045
CGGTATT1700.035.73283424
CGTCATA1600.035.1538324
CTCGGGG1350.034.9975913
TAAGCCG1550.034.8363113
TATGCCG2850.034.7344543
ATTCGGT1750.034.71189521
Produced by FastQC (version 0.11.5)