FastQCFastQC Report
Mon 6 Jun 2016
ERR1378117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596299
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCACAATCTCGTATGCCGTC21010.352340017340294Illumina Paired End PCR Primer 2 (96% over 30bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA14780.24786223018988798No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCACAATCTCGTATGCC11780.1975518992988417Illumina Paired End PCR Primer 2 (96% over 33bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA10300.17273213605925886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG1950.041.5301816
TAAGCCG1950.041.5301813
AGCCGAT2000.040.4919215
TGCCGTC2250.040.00879745
TATGCCG2250.039.99537743
GACGTCA2050.039.5076322
ATGCCGT2300.039.12591644
CGTCATA2100.038.56697524
AAGCCGA2100.038.5637414
CGATGAC2100.038.5637418
ACGCCCG356.2517847E-638.56373612
ATGACGT2150.037.6669120
TGACGTC2200.036.8139321
CCGATGA2200.036.8108417
GGCGGTC1500.035.9958435
TCGGGGA1500.035.9928214
AATTCGG1500.035.9928220
ACGTCAT2300.035.21332623
GATGACG2300.035.2103719
GCGGTCT1600.033.746136
Produced by FastQC (version 0.11.5)