FastQCFastQC Report
Mon 6 Jun 2016
ERR1378116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767929
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGACGGAATCTCGTATGCCGTC19690.25640391234085447Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGACGGAATCTCGTATGCC11130.1449352739641295Illumina Paired End PCR Primer 2 (96% over 33bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA11000.14324240912896896No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA8790.11446370693123974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2450.041.3239644
TATGCCG2500.040.49748243
TGCCGTC2550.039.7163445
TAAGCCG1200.039.37254713
GACGTCA1250.037.7976522
TCGGTAT1550.037.7395923
AATTCGG1500.037.49766520
GGCGGTC1600.036.56022635
TTCGGTA1550.036.28806722
TCTCGAC250.002107317635.9977620
GCGGTCT1600.035.15406436
AGGCGGT1600.035.15406434
CCGATGA1350.034.9978217
CGTCATA1350.034.9978224
CGGGGAA1650.034.08878715
CGGTATT1650.034.08878724
GAATTCG1650.034.08878719
GCCGATG1400.033.74790216
ATGACGT1400.033.74790220
CGATGAC1400.033.74790218
Produced by FastQC (version 0.11.5)