FastQCFastQC Report
Mon 6 Jun 2016
ERR1378115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568688
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCGAGAAATCTCGTATGCCGTC25190.442949385251667Illumina Paired End PCR Primer 2 (96% over 30bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA20240.3559069296345272No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA15400.2707987508088794No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGAGAAATCTCGTATGCC14900.26200658357482487Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAT2300.044.01580423
ATGACGT2150.042.9011920
TTCGGTA2400.042.18181222
CGGTATT2400.042.18181224
AGCCGAT2200.041.92616715
GCCGATG2200.041.92616716
ATGCCGT2850.041.83646444
GCGGTCT2450.041.3209636
GAATTCG2450.041.3209619
TGACGTC2250.040.99447321
ACGTCAT2250.040.99447323
GGCGGTC2500.040.4945435
CGGTCTC2500.040.4945437
TATGCCG3000.040.49453743
CCGATGA2300.040.10328717
GACGTCA2300.040.10328722
TAAGCCG2300.040.10328713
GATGACG2350.039.25002319
CGTCATA2350.039.25002324
TCCTCGG2300.039.15270211
Produced by FastQC (version 0.11.5)