FastQCFastQC Report
Mon 6 Jun 2016
ERR1378112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714450
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA18050.2526418923647561No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACAAACATCTCGTATGCCGTC14840.20771222618797677Illumina Paired End PCR Primer 2 (96% over 31bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA12500.17495975925537124No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACAAACATCTCGTATGCC10840.15172510322625796Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1900.040.2904845
CGATGAC1900.040.25664518
GGCGGTC1650.039.5390635
GACGTCA1950.039.22442222
TGACGTC1950.039.22442221
GCGGTCT1700.038.37614436
GCCGATG2000.038.24381316
TAAGCCG2000.038.24381313
TCGGTAT1750.037.27968223
ATGCCGT2150.035.5781344
CGTCATA2150.035.5781324
AGCCGAT2150.035.57563815
CCGATGA2150.035.57563817
AGGCGGT1850.035.2645734
AATTCGG1850.035.2645720
TATGCCG2200.034.7695443
ACGTCAT2200.034.76710523
CTCGGGG1700.034.406213
CGGTATT1900.034.33895524
TCGGGGA1900.034.3365514
Produced by FastQC (version 0.11.5)