FastQCFastQC Report
Mon 6 Jun 2016
ERR1378110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651364
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCTCCATCTCGTATGCCGTC21960.33713868129033847Illumina Paired End PCR Primer 2 (97% over 35bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA14630.22460559687056697No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCTCCATCTCGTATGCC13020.1998882345355285Illumina Paired End PCR Primer 2 (97% over 38bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA10710.16442419292438637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCA1600.043.5895122
TAAGCCG1600.043.5895113
CGTCATA1600.043.5895124
TGCCGTC1750.042.45051645
CCGATGA1650.042.26861617
TTCGGTA1200.041.24598722
TCGGTAT1200.041.24598723
TGACGTC1700.041.0254221
AGCCGAT1750.039.85326815
GCCGATG1750.039.85326816
ATTCGGT1250.039.5961521
ATGACGT1800.038.7462320
CGATGAC1800.038.7462318
AATTCGG1300.038.07322320
TATGCCG2000.037.12138743
AAGCCGA1950.035.76575514
ACGTCAT1950.035.76575523
GATGACG1950.035.76575519
AGGCGGT1400.035.35370334
CTCGGGG1150.035.2139713
Produced by FastQC (version 0.11.5)