FastQCFastQC Report
Mon 6 Jun 2016
ERR1378107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792300
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA21020.2653035466363751No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGGCGATCTCGTATGCCGTC13770.17379780386217342Illumina Paired End PCR Primer 2 (96% over 30bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA13290.16773949261643317No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGGCGATCTCGTATGCC7950.10034078000757289Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT2150.041.85318815
CCGATGA2150.041.85318817
CGATGAC2150.041.85318818
TAAGCCG2150.041.85318813
CGTCATA2200.040.90197424
GCCGATG2250.039.99304616
AAGCCGA2250.039.99304614
TATGCCG2200.039.87942543
GGCGGTC2150.039.7605335
GACGTCA2350.039.24849322
ATGCCGT2250.038.99321744
GCGGTCT2200.038.85687636
ATTCGGT2200.038.85687621
AATTCGG2200.038.85687620
TGACGTC2350.038.2912121
CGGTATT2300.038.1455424
TCGGTAT2250.037.99339323
ACGTCAT2400.037.49347723
GATGACG2400.037.49347719
TGCCGTC2350.037.34571545
Produced by FastQC (version 0.11.5)