FastQCFastQC Report
Mon 6 Jun 2016
ERR1378106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905298
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA19340.2136313125622723No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTATATCTCGTATGCCGTC19090.21086979094176722Illumina Paired End PCR Primer 2 (97% over 34bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA14470.15983687139483352No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTATATCTCGTATGCC12130.13398902902690607Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2300.041.08181443
TAAGCCG2100.040.70918713
TGACGTC2100.039.63789421
CGTCATA2100.039.63789424
ACGTCAT2150.038.71608423
GACGTCA2200.037.8361722
CCGATGA2350.037.3357517
AGCCGAT2300.037.1692615
GATGACG2300.037.1692619
TCGGGGA1900.036.7059314
AAGCCGA2350.036.37842614
TGCCGTC2600.036.36771845
GCCGATG2400.035.6205416
CGATGAC2500.035.09560418
ATGCCGT2700.034.99561744
GCGGTCT2000.034.87063236
TCGGTAT2000.034.87063223
GGCGGTC2050.034.0201335
ATGACGT2450.033.97533820
TTCCTCG1800.032.5138910
Produced by FastQC (version 0.11.5)