FastQCFastQC Report
Mon 6 Jun 2016
ERR1378104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810488
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCCGCATCTCGTATGCCGTC27110.33448983822092365Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCCGCATCTCGTATGCC17940.22134812606725823Illumina Paired End PCR Primer 2 (97% over 36bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA15630.1928467787308387No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA11520.14213658931409226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA1800.042.49324424
TTCGGTA1650.040.90258422
TGCCGTC3050.040.5698245
GATGACG1950.040.37819319
ATTCGGT1700.039.6995721
TCGGTAT1700.039.6995723
AGCCGAT2000.039.36873615
GCCGATG2000.039.36873616
TAAGCCG2100.038.56529213
ATGCCGT3250.038.07086644
AAGCCGA2150.037.66842714
GGCGGTC1800.037.49403835
CGATGAC2100.037.49403418
TGACGTC2050.037.3111421
TATGCCG3350.036.9344243
TTCCTCG1650.036.8441510
GACGTCA2100.036.42277522
ACGTCAT2150.035.57573723
GCGGTCT1900.035.52066836
AATTCGG1900.035.52066820
Produced by FastQC (version 0.11.5)