FastQCFastQC Report
Mon 6 Jun 2016
ERR1378103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660925
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCGAAATCTCGTATGCCGTC17200.26024132844119985Illumina Paired End PCR Primer 2 (96% over 33bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA15330.23194764912811588No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA10600.16038128380678596No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCGAAATCTCGTATGCC9490.1435866399364527Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG1850.042.56048213
AGCCGAT1950.040.37789515
GCCGATG2000.039.36844616
ACGTCAT2050.038.40823723
CGTCATA2050.038.40823724
GCGGTCT1300.038.07058336
ATGCCGT1850.037.69642644
CGATGAC2100.037.4937618
TATGCCG1900.036.7044243
CCGATGA2150.036.6218117
GACGTCA2150.036.6218122
GATGACG2150.036.6218119
TGACGTC2200.035.78949721
AAGCCGA2200.035.78949714
GGCGGTC1450.035.68371635
TTCGGTA1450.035.68371622
TCGGTAT1400.035.3512623
ATGACGT2250.034.99417520
TGCCGTC2000.034.87447445
AGGCGGT1500.032.9945134
Produced by FastQC (version 0.11.5)