FastQCFastQC Report
Mon 6 Jun 2016
ERR1378101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788844
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCTTAATCTCGTATGCCGTC21760.2758466819802141Illumina Paired End PCR Primer 2 (96% over 30bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA18480.23426685124054947No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA12280.1556708297204517No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCTTAATCTCGTATGCC9840.12473949221899387Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATT1900.041.44182224
TGCCGTC2550.039.70812245
AGGCGGT2000.039.3697334
TATGCCG2600.038.93709643
TCGGTAT2100.038.56626523
GGCGGTC2050.038.40949235
TTCGGTA2050.038.40949222
ATGCCGT2650.038.20243544
CTCGGGG1600.037.9636713
AGCCGAT2350.037.33542615
TCGGGGA2150.036.62300514
AATTCGG2150.036.62300520
GCCGATG2400.036.55760616
CGTCATA2450.035.8115324
ATTCGGT2200.035.79066521
CCTCGGG1700.035.7305112
ATGACGT2550.035.289420
GATGACG2500.035.09530319
CGATGAC2500.035.09530318
GCGGTCT2250.034.99531636
Produced by FastQC (version 0.11.5)