ERR1378099.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1378099.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686706
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCCGCAAATCTCGTATGCCGTC	1668	0.24289870774392536	Illumina Paired End PCR Primer 2 (96% over 30bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA	1333	0.19411509437808902	No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA	1003	0.14605959464457863	No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCGCAAATCTCGTATGCC	979	0.14256464920941422	Illumina Paired End PCR Primer 2 (96% over 33bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGACGTC	150	40.495937	21
AGCCGAT	150	40.49299	15
TAAGCCG	150	40.49299	13
TGCCGTC	185	40.145725	45
CGATGAC	165	38.177986	18
ATGACGT	160	37.96494	20
CGTCATA	160	37.96494	24
GCCGATG	160	37.962173	16
AAGCCGA	165	36.811806	14
GCGGTCT	150	35.99377	36
CGGGGAA	150	35.99377	15
GGCGGTC	150	35.99377	35
GACGTCA	170	35.731712	22
TATGCCG	210	35.35102	43
ATTCGGT	160	35.15272	21
AATTCGG	155	34.835217	20
ATGCCGT	215	34.528904	44
TTCGGTA	160	33.746613	22
CTCGGGG	135	33.327564	13
ACGTCAT	185	32.83454	23