FastQCFastQC Report
Mon 6 Jun 2016
ERR1378098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734373
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATCCTAATCTCGTATGCCGTC29440.4008861981581567Illumina Paired End PCR Primer 2 (96% over 33bp)
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA16080.21896229845051493No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCCTAATCTCGTATGCC15490.2109282340173182Illumina Paired End PCR Primer 2 (97% over 36bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA11570.1575493652408245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT1750.042.4240115
GGCGGTC1850.041.3469135
GACGTCA1800.041.24556722
TGCCGTC4000.040.512245
TCGGTAT1950.040.38027623
GCGGTCT1900.040.2588336
CGGGGAA1900.040.2588315
AATTCGG1900.040.2588320
CGGTATT1900.040.2588324
GCCGATG1850.040.13082516
ATGCCGT4100.039.5079544
TATGCCG4150.039.0319543
ACGCCCG356.249813E-638.56728712
TCGGGGA2050.038.41050714
AGGCGGT2000.038.2458934
CCGATGA1950.038.07283417
TTCGGTA2050.037.31306522
GAATTCG2050.037.31306519
TCCTCGG1700.037.07251411
ACGTCAT2050.036.21562223
Produced by FastQC (version 0.11.5)