FastQCFastQC Report
Mon 6 Jun 2016
ERR1378097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692583
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA16380.23650594946742848No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACTGATATCTCGTATGCCGTC11950.17254249671158547Illumina Paired End PCR Primer 2 (96% over 31bp)
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA11400.16460121025205643No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTGATATCTCGTATGCC8630.12460600390133746Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG1200.041.2390813
TCGGTAT1400.040.1795423
CGTCATA1250.039.60095624
TATGCCG1600.039.37026243
TTCGGTA1450.038.79403722
GCCGATG1350.038.32595416
ATGCCGT1650.038.17722344
ACGTCAT1350.036.6675523
AATTCGG1550.036.28857420
TGCCGTC1750.036.0008745
CGGTATT1600.035.15709324
CTCGGGG1350.034.99073413
GGCGGTC1550.034.83703235
CCGATGA1500.034.49335517
AAGCCGA1500.034.49086814
GACGTCA1450.034.13875622
GATGACG1450.034.13628819
ATTCGGT1650.034.09173221
TCGGGGA1650.034.08188614
CGATGAC1550.033.3854918
Produced by FastQC (version 0.11.5)