FastQCFastQC Report
Mon 6 Jun 2016
ERR1390786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404567
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC6160.15226155371051026Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAAG250.002108196735.9906139
CACCCGG250.002108196735.9906113
AGATCGG1050.029.99217217
CGTATGC300.00514992929.99217244
ACCCGGC300.00514992929.99217214
CGCAAGA300.00514992929.99217240
TCGGAAG1001.8189894E-1229.24236920
ATCGGAA1001.8189894E-1229.24236919
GATCGGA1105.456968E-1226.58397118
GAGATCG1201.6370905E-1124.36863916
CGAGATC1305.0931703E-1122.49413115
CCTTCGT754.5413824E-521.0309121
CGTGAGC754.5413824E-521.0309121
CGGTTCA1401.364242E-1020.88740529
CCGAGAT1401.364242E-1020.88740514
CCCGGCC550.003938144520.4492115
GCGGTTC1452.1827873E-1020.16714928
CCGAGAC1452.1827873E-1020.16714945
CGGAAGA1503.43789E-1019.49491121
AGCGGTT1555.329639E-1018.86604527
Produced by FastQC (version 0.11.5)