FastQCFastQC Report
Mon 6 Jun 2016
ERR1390785.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390785.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698814
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT1101.4915713E-1024.5806052
CCTTCGT1052.3246685E-923.6051831
CGGGATT1358.367351E-1121.6619814
TCGTTGG1257.385097E-1021.630934
AGCGCTC654.2359834E-420.7691977
ACAGCGT550.003938316420.45158627
AGATCGG1109.9928E-820.45012317
GATCGGA1109.9928E-820.45012318
CGTTGGA1351.9172148E-920.035825
TTCGTTG1351.9263098E-920.0286393
GAAACGG1800.019.99996810
ACGGGAT1351.9663275E-919.99567213
CGCTCCA807.557669E-519.6874689
ACCACGC1151.5931255E-719.5651849
AACGGGA1403.094101E-919.27878412
AAACGGG1801.0913936E-1118.7499711
CTACTCG600.00651882418.74460232
TCCGTTC600.00651882418.74460233
GGAAACG1608.1490725E-1018.2812219
CGGAAGA1253.8394865E-717.9948221
Produced by FastQC (version 0.11.5)