FastQCFastQC Report
Mon 6 Jun 2016
ERR1390775.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390775.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870007
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC708.1672624E-1032.1323928
GATCGGA1000.031.4915518
TCTCGTA751.70985E-929.98850841
CTCGTAT803.4069672E-928.11422542
AGATCGG1150.027.38395717
ATCTCGT1004.5474735E-1126.98965540
GACCGCA856.502887E-926.46044729
TATCTCG957.4396667E-1026.04264839
CGCAGAG957.4396667E-1026.04264832
CGCCCGG901.19362085E-824.99329413
CGTATGC901.195076E-824.9904244
CCGCAGA901.195076E-824.9904231
CGCGCCC551.3681847E-424.54451411
ATCGGAA1301.8189894E-1224.22426819
TCGTATG952.1225787E-823.67513543
ACCGCGC702.701615E-522.5004339
ACGCCCG602.4732467E-422.49137912
GCGCCCG500.002269217522.49137912
ACCGCAG1101.00175384E-720.44670930
TAACACG550.003944525520.44670920
Produced by FastQC (version 0.11.5)