FastQCFastQC Report
Mon 6 Jun 2016
ERR1390773.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390773.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899114
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT554.1711082E-628.62974529
CCGGTAG554.1711082E-628.62974531
CCAAACG551.3700264E-424.53978220
CGTATGC651.5260004E-524.2251744
AGACCGG651.5260004E-524.2251728
CGGTAGC651.5260004E-524.2251732
GATCGGA751.7003786E-623.99445318
ACCGGTA702.7069751E-522.49480230
AGATCGG802.979532E-622.494817
TCGGAAG802.979532E-622.494820
ATCGGAA802.979532E-622.494819
CTCGTAT754.6094035E-520.99514842
TCTCGTA754.6094035E-520.99514841
ACGCCCG807.574101E-519.68295112
CCGGCTA1454.782123E-918.61638616
CCGCCAC750.001100536418.0229024
GTATGCC952.8234711E-416.57511545
TCGTATG952.8234711E-416.57511543
CGGCTAA1652.3626853E-816.35985417
CACGCCC1703.405148E-815.88133211
Produced by FastQC (version 0.11.5)