FastQCFastQC Report
Mon 6 Jun 2016
ERR1390764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370561
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA403.4541517E-739.3686218
ATCGGAA403.4541517E-739.3686219
AGATCGG501.9793297E-631.494917
CGTATGC602.4177825E-729.99109344
CGGCATA602.4177825E-729.99109332
GACCGGC602.4177825E-729.99109329
AGACCGG654.865269E-727.68782428
ACCGGCA654.871017E-727.68408830
CTCGTAT654.871017E-727.68408842
CCGGCAT654.871017E-727.68408831
TCTCGTA654.871017E-727.68408841
TCGTATG654.871017E-727.68408843
TCGGAAG608.190847E-626.24220720
GAGACCG751.6948616E-623.99611527
ATCTCGT751.6968534E-623.99287640
GTATGCC855.029953E-621.17018345
CCGAGAT754.5975645E-520.996614
CGGAAGA908.1233775E-620.0291961
GCGGTTC908.2380175E-619.9967618
CGAGATC807.5547505E-519.6843115
Produced by FastQC (version 0.11.5)