FastQCFastQC Report
Mon 6 Jun 2016
ERR1390761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950339
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCG654.886151E-727.68304812
TCGGAAG856.501068E-926.46173720
AGATCGG801.05626896E-725.30536717
GATCGGA851.8980973E-723.81681618
CGCCCGG1204.5292836E-1022.49365813
CCGAGAC1305.0931703E-1122.49247645
ATCGGAA1056.123264E-821.42253319
GCGGTTC1301.2314558E-920.76228528
ATCTTCG1251.724402E-819.8414025
CGTATAA951.3174435E-518.94402310
GCCCGGC2001.8189894E-1217.99492614
CGGTTCA1253.8449616E-717.99398229
CCGGCTA1802.0736479E-1017.4950716
GAGCGGT1205.1876887E-616.86935626
TCGTATA1104.6431687E-516.3607469
CACGCCC1401.2571381E-616.06774511
AAGAGCG1301.1094997E-515.57171424
AGAGCGG1301.1094997E-515.57171425
ACCACGC1908.007191E-915.3920199
CGGCTAA1908.018105E-915.39039917
Produced by FastQC (version 0.11.5)