FastQCFastQC Report
Mon 6 Jun 2016
ERR1390759.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390759.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712169
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACGC1201.10685505E-820.6194029
CGTATAA600.006434218518.7871234
TCGTTGG1459.436735E-817.1027584
CGGGATT1205.180904E-616.87041914
GGCGGAG1358.601801E-716.6609731
CTTCGTT952.7752714E-416.611772
CCTTCGT1104.5556968E-516.3960321
CCACGCC1855.6261342E-915.80651910
CGTTGGA1705.7438956E-714.5886755
ACTAGAC2702.1827873E-1114.1628219
TTCGTTG1604.9179325E-614.0903413
ACGGGAT1301.9289464E-413.84239513
AAACGGG2154.0756277E-813.60095811
GCTAATT4650.013.54470919
CGCCACC1504.1846793E-513.5276795
GCCCGGC1352.6558238E-413.32971314
GAAACGG2205.5082637E-813.29184410
CACGCCC1250.002254655112.59657911
GGAAACG2003.6677757E-612.3716419
AGGCGGA1300.003027297612.11124530
Produced by FastQC (version 0.11.5)