FastQCFastQC Report
Mon 6 Jun 2016
ERR1390758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617318
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC17730.28721015748771295Illumina Paired End PCR Primer 2 (100% over 35bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGCAGATCTCGTATGCC9540.15453947560252576Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCA1550.031.92674632
TCTCGTA1550.031.9241641
CGTATGC1600.030.92652944
CTCGTAT1600.030.92652942
GACCGAT1650.029.9917929
ACCGATG1650.029.9917930
TCGTATG1650.029.98936343
AGATCGG3500.029.56333717
GATCGGA3600.028.74213218
ATCGGAA3600.028.74213219
CCGATGC1750.028.27797531
CCGAGAT3850.028.04427314
CGAGATC3850.028.04427315
CCGAGAC4150.026.55684545
ATCTCGT1900.026.04550240
AGACCGA1900.026.04550228
TCGGAAG3900.025.95233220
GAGACCG2000.025.86792227
GAGATCG4250.024.34627716
GTATGCC2100.023.5630745
Produced by FastQC (version 0.11.5)