FastQCFastQC Report
Mon 6 Jun 2016
ERR1390757.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390757.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470552
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9160.19466498920416872Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAC2050.030.72401445
AGATCGG2150.030.34124217
GATCGGA2150.030.34124218
CGAGATC2150.030.34124215
ATCGGAA2300.028.36246519
GAGATCG2300.028.36246516
CCGAGAT2350.027.7590114
TCGGAAG2400.027.18069620
CGGAAGA2550.025.58183121
GCGGTTC2750.023.72133428
GAGCGGT2750.023.72133426
AGCGGTT2800.023.29773927
GGCGACA500.002265930622.4943729
AGAGCGG2950.022.11310825
CGGTTCA3000.021.74455629
AAGAGCG3050.021.38808824
TGGGCGA550.00393884220.44942727
CCCGTCT706.902323E-419.3157791
GTTCAGC3500.019.28088831
GAATCGC600.00651432118.74530812
Produced by FastQC (version 0.11.5)