FastQCFastQC Report
Mon 6 Jun 2016
ERR1390754.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390754.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734270
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG754.7293724E-1132.98901417
GATCGGA801.0004442E-1030.927218
GGCGGTG300.005156162729.9879737
ATCGGAA903.965397E-1027.49084519
CGCCCGC507.042504E-527.062891
CGTATGC450.001229003224.98997344
CTCGTAT450.001229003224.98997342
CCGTGTC602.473035E-422.49097831
CGTGAGC1055.9519152E-821.4784811
CGCCCGG654.2457535E-420.76231613
TCGGAAG1201.108674E-820.6167320
CCGAGAC1201.108674E-820.6167345
AAATCGC550.0038809920.5035885
GACCGTG550.00394435620.44634229
TCTCGTA550.00394435620.44634241
CGAGATC1151.5989826E-719.55870215
TTCGTTG1402.9940566E-919.3306353
CGTTGGA1555.16593E-1018.9162145
AGACCGT600.006520829618.7437628
TCGTATG600.00652339418.74248143
Produced by FastQC (version 0.11.5)