FastQCFastQC Report
Mon 6 Jun 2016
ERR1390751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832651
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT802.9815383E-622.49267642
AGACCGT754.610102E-520.99442328
CGTATGC754.6122357E-520.99316244
ACGGGTT550.003941720.44911413
CCGTGTA908.265717E-619.99468831
TCTCGTA851.20694414E-418.52337841
TCGTATG851.20694414E-418.52337843
GAGACCG901.8686896E-417.49535227
GACCGTG1053.1178555E-517.13830629
ATCTCGT952.8238993E-416.57454540
AGATCGG952.8238993E-416.57454517
CGTTGGA2009.0221874E-1015.7789825
CTTCGTT1451.776325E-615.5429892
CGTATAA1056.0382934E-414.99871910
CCTTCGT1553.5526173E-614.54021551
TCGTATA1108.590393E-414.3169589
TTCGTTG2052.12749E-814.2919683
CGGAAGA950.005315575714.2341061
GGAAACG2102.988054E-813.9273829
CATCTCG1150.001203701613.69201639
Produced by FastQC (version 0.11.5)