FastQCFastQC Report
Mon 6 Jun 2016
ERR1390749.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390749.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976735
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC11200.11466774508950739Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCAT300.00515140929.99460811
TCTCGTA803.4033292E-928.11850441
GATCGGA1850.027.96794518
AGATCGG2000.025.8703517
TCGGAAG2200.025.56227720
CTCGTAT901.19343895E-824.99422642
ATCGGAA2200.024.54104219
CGTATGC952.1198503E-823.6787444
CCGAGAC2550.022.93587945
GACCGTC1003.651803E-822.49480229
AGACCGT1056.1170795E-821.42471928
TATCTCG1056.120354E-821.42362439
TCGTATG1056.120354E-821.42362443
ACCGTCA1056.120354E-821.42362430
CGAATGA550.003941389720.44982137
GAGATCG2650.019.52479216
CGGAAGA2800.019.2812621
TCTCGTT600.006516569818.7466310
CGTCATG1202.5017653E-718.7456732
GCGGTTC3050.017.70173628
Produced by FastQC (version 0.11.5)