FastQCFastQC Report
Mon 6 Jun 2016
ERR1390738.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390738.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731772
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA352.8249898E-432.13197330
CCGCTAC352.8249898E-432.13197331
CGTATGC406.176206E-428.11547744
CGCTACA507.171417E-526.99085832
AGACCGC450.001228062424.99324228
GACCGCT450.001228551724.99153529
GAGACCG550.00394135820.44901727
CTTCGTA807.5659686E-519.684877
TCGTTGG1053.062716E-517.1734284
CCCGGGT850.002569770715.87697431
TGAGCCG1351.5850603E-514.99492239
CGTTGGA1251.356248E-414.4286445
TTCGTAT950.005378308214.2086288
AGCCGAG1605.0377894E-614.05773841
CCGAGAT1605.0377894E-614.05773843
TCTTCGT1150.001183159213.7228966
GAGCCGA1555.822637E-513.06009240
ATCTTCG1250.002216824812.6250645
CGTGAGC1300.002978121612.1378211
TTCGTTG1300.002979650212.13699153
Produced by FastQC (version 0.11.5)