FastQCFastQC Report
Mon 6 Jun 2016
ERR1390734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193126
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT2850.018.9835831
CGTAGAT750.001113102817.99410832
CCGTAGA800.001715266816.86947631
ATCTCGT1104.6536297E-516.3582840
GACCGTA850.002571771415.87715429
CGGGATT3250.015.22682514
CTTCGTT3500.014.8139742
ACGGGAT3250.014.53469713
TTCGTTG3900.014.4506443
ATCGTGC1108.463574E-414.3468595
TCGTATG1108.6155283E-414.313169543
CGTTGGA3800.014.2389895
ACCGTAG950.00538976214.20587530
CGCGCCC1453.0521638E-513.9918914
TCGTTGG3900.013.8723014
AGCGAGA1951.8387436E-713.84162137
GGCGACA1951.8387436E-713.84162129
CGAGGCG1000.007566835313.49650516
ATCGCGC1200.001632460713.1512885
AAACGGG4650.013.06143111
Produced by FastQC (version 0.11.5)