FastQCFastQC Report
Mon 6 Jun 2016
ERR1390732.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390732.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166941
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGA406.175686E-428.11760932
CGTATGC406.1772345E-428.11640444
CCGATAG500.002268091322.49408731
GACCGAT500.002268091322.49408729
TATCTCG754.6120418E-520.9944839
TCTCGTA550.0039425720.44917141
CTCGTAT600.006520441718.74507142
ACCGATA600.006520441718.74507130
TTCACGC850.002569697615.878180515
ATGCCCG850.002570432415.87749912
GTATAGG900.00376053714.996057531
GTATGCC900.00376160914.99541545
CACTCGT1000.007478893313.5196251
ACGGAAG1250.00225600212.59668824
GAGCCGA2156.013979E-712.55483840
CGTGAGC1801.6682228E-512.5181711
CGGCTAA1300.003027500612.11220117
GCCCGGC2054.840156E-612.06999714
CACGCCC1751.805324E-411.57086711
CCGGCTA1650.001435856610.90622416
Produced by FastQC (version 0.11.5)