FastQCFastQC Report
Mon 6 Jun 2016
ERR1390731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680451
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC14580.21426965350921667Illumina Paired End PCR Primer 2 (100% over 35bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGGCATATCTCGTATGCC7830.11507073984754229Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGT401.5627236E-533.74024612
CGTATGC1250.032.39063644
CACGCTC300.00515307729.99133111
ATCTCGT1350.029.9913340
CTCGTAT1350.029.9913342
TATCTCG1450.029.47423639
TCTCGTA1550.026.1214841
AGATCGG2450.024.78875217
GAGATCG2450.024.78875216
GATCGGA2550.023.81664318
CGAGGCA1700.023.81664332
TCGTATG1700.023.81664343
ATCGGAA2550.023.81664319
GACCGAG1750.023.1361729
AGACCGA1750.023.1361728
ACCGAGG1800.022.49349830
GTATGCC1800.022.49349845
CCGAGGC1800.022.49349831
CTTCGTA802.9792627E-622.4934967
CGAGATC2850.022.09887515
Produced by FastQC (version 0.11.5)