FastQCFastQC Report
Mon 6 Jun 2016
ERR1390727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448822
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC4600.10249051962693452Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATCG301.1263162E-437.5703475
CGGAACA250.002108277835.99117732
AACGCAC250.002108277835.9911779
GATCGGA900.034.9914218
AGATCGG950.033.14976517
ATCGGAA950.033.14976519
CCGGAAC352.8217107E-432.1349831
CTCGTAT352.8235564E-432.131442
TCGGAAG1000.031.48877120
ATCTCGT300.005150124429.99264340
CGTATGC300.005152931429.98930444
TCGTATG300.005152931429.98930443
GCGGTTC1150.027.3845928
AGCGGTT1150.027.3845927
CCGAGAC1150.027.38153845
CGGAAGA1200.026.2406421
AAGAGCG1300.025.95228224
CGAGATC1251.8189894E-1225.19382315
AGAGCGG1251.8189894E-1225.19382325
CGGGATT450.001227142224.99387214
Produced by FastQC (version 0.11.5)