FastQCFastQC Report
Mon 6 Jun 2016
ERR1390724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668945
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA751.6993126E-623.9944217
CGCCACC702.6699321E-522.5335085
ACGCCCG500.00226668922.49476812
CGCCTGC550.003940148320.4497931
CCGGCTA1051.4469424E-619.28122916
CCCGCCA951.2994016E-518.9727463
GATCCGC600.006516461718.7456427
TATACCC750.001111296617.99581535
CGCCCGG800.001712498416.87107513
TCGTTGG952.786933E-416.6023964
CCGCCAC1004.119255E-415.7722744
ACTAGAC2750.015.5418419
CCCGGCT1209.761792E-514.99651115
GCCCGGC1351.5830668E-514.99651114
CGCTTGA1656.90498E-613.63523110
TAGACAG4650.013.54523621
ATCGCTT1504.2686115E-513.49887858
CTAGACA4550.013.34854320
GAAGCAT4100.013.16766927
TCGCTTG1555.8072423E-513.0634319
Produced by FastQC (version 0.11.5)