FastQCFastQC Report
Mon 6 Jun 2016
ERR1390723.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390723.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686243
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA550.00393682720.4528589
CCAACGA706.911806E-419.3136796
CCCTATA851.20565186E-418.52518325
CGTTGGA1002.0193478E-518.02615
TCCAACG750.001098865318.02615
TGAGCCG1305.788024E-717.30374145
GCAACTC1004.1229074E-415.7705353
ATCTTCG900.003715868815.021755
CCTTCGT1209.6294105E-515.0206541
AAACGGG1209.7559576E-514.99766711
CGGGATT900.003757571614.99657514
ACCACGC1150.001201662513.6945219
TCTGTCG1000.007474947313.5195756
CTTCGTA1000.00755106713.4988857
AACGGGA1000.007558350513.49691812
TCGTTGG1403.561522E-412.87484554
GAAACGG1403.6056698E-412.85608110
GGAAACG1403.6056698E-412.8560819
CCACGCC1250.002251314212.59895910
GTATGAT1454.8534403E-412.41095827
Produced by FastQC (version 0.11.5)