FastQCFastQC Report
Mon 6 Jun 2016
ERR1390720.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390720.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655204
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA403.4620825E-739.36498618
ATCTCGT751.7062121E-929.99236740
ACCGGTA751.7062121E-929.99236730
CGTATGC751.7062121E-929.99236744
GACCGGT751.7062121E-929.99236729
TCTCGTA751.7062121E-929.99236741
CCGGTAT751.7062121E-929.99236731
CTCGTAT852.0372681E-1029.1102442
AGATCGG554.169593E-628.6290817
TCGTATG803.3996912E-928.11784643
GTATGCC903.965397E-1027.49300645
CGGTATT901.19234755E-824.99364332
ATCGGAA651.5254547E-524.22460619
CATCTCG1003.648165E-822.49427639
GCGTGAA500.002266927422.49427634
GAGACCG1056.114169E-821.4231227
AGACCGG1056.114169E-821.4231228
TCGGAAG754.607779E-520.99465820
AGCGGTT1109.962423E-820.4555877
GGCGCCC550.00394056120.44934323
Produced by FastQC (version 0.11.5)