FastQCFastQC Report
Mon 6 Jun 2016
ERR1390717.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390717.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656454
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG554.1716285E-628.627317
GATCGGA608.204694E-626.24169318
CTTCGTT751.6702779E-624.0418452
TCGGAAG802.9797084E-622.49287820
ATCGGAA702.707324E-522.49287819
CCTTCGT955.432448E-721.3529551
ACGGTGG951.3145611E-518.9471447
GGGCACG600.006442459818.7826943
CGGAAGC600.00651949318.74406625
GCACGGT851.18538934E-418.565975
AGAGCGG851.2063665E-418.52354825
CGTTGGA1406.319351E-817.7134535
TCGTTGG1305.6733734E-717.3365464
CACGGTG1053.0569667E-517.176686
GGCACGG952.7814714E-416.6065864
AAGAGCG952.8238547E-416.573724
AGCGGTT952.8238547E-416.573727
GAGCGGT952.8238547E-416.573726
CGGGATT1257.639112E-616.19487214
CGCCACC1004.1016596E-415.78107555
Produced by FastQC (version 0.11.5)