FastQCFastQC Report
Mon 6 Jun 2016
ERR1390712.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390712.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568810
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7300.12833810938626256Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1050.032.1334418
CGGGAAA300.005152476529.9912145
AGATCGG1200.028.1167617
TCGTATA507.166431E-526.9920889
TCGGAAG1300.025.9539320
ATCGGAA1300.025.9539319
TTCGTAT651.5254514E-524.223678
CGGAAGA1400.024.1000821
CCGAGAC1400.024.1000845
CGGTTCA1450.023.26904129
GAGCGGT1450.023.26904126
CCGAGAT1450.023.26904114
AGAGCGG1450.023.26904125
AGCGGTT1551.8189894E-1221.76781527
CGAGATC1551.8189894E-1221.76781515
GCGGTTC1601.8189894E-1221.0875728
CTTCGTA754.6077785E-520.9938477
CGTATAA654.242988E-420.76314510
GAGATCG1653.6379788E-1220.44855116
AAGAGCG1757.2759576E-1219.28006424
Produced by FastQC (version 0.11.5)