FastQCFastQC Report
Mon 6 Jun 2016
ERR1390709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606120
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA551.3708748E-424.53564518
CTTCGTT702.6555555E-522.5486742
AGATCGG602.4723218E-422.49100717
ATCGGAA706.9970527E-419.27800819
GTGGCGA600.006522060418.74250636
TCTCGAT1002.043815E-518.000239
CTACTCG1153.4563745E-617.60165832
TCGGAAG800.001714163616.86825820
TACTCGG1205.1846637E-616.86825633
CGTTGGA900.003689509415.0374225
TCGTTGG900.00369764215.0324484
CGGAAGA900.003761183714.99400521
CTCGATC1251.3785964E-414.40018510
CCGAGAC1108.60609E-414.31245945
ATCGCTT950.005371674414.2107098
GCGGTTC950.005386397214.20484728
GCTACTC2905.456968E-1213.95993531
TCGCTTG1000.007544963613.5001739
CGGTTCA1000.00756558313.49460529
AGCGGTT1000.00756558313.49460527
Produced by FastQC (version 0.11.5)