FastQCFastQC Report
Mon 6 Jun 2016
ERR1390706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695353
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCTA651.4049874E-831.14682831
CGTATGC602.4214387E-729.9932444
CGCCTAT702.8891009E-828.9220532
CTCGTAT654.8783295E-727.6860742
TCTCGTA654.8783295E-727.6860741
TCGTATG654.8783295E-727.6860743
AGACCGC755.644688E-826.99391728
GATCGGA709.316973E-725.7084918
TCGGAAG709.316973E-725.7084920
GACCGCC801.05483196E-725.30679729
GAGACCG801.05483196E-725.30679727
ACCGCCT851.8955325E-723.81816130
ATCTCGT955.53724E-721.31098740
AGATCGG855.037313E-621.17169817
ATCGGAA855.037313E-621.17169819
AATCTCG951.31716715E-518.943139
CGGAAGA1358.436873E-716.6917151
GAGCCGA952.822192E-416.57521240
CCGAGAC1257.633798E-616.1963525
GCGGTTC1401.2549608E-616.0689628
Produced by FastQC (version 0.11.5)