FastQCFastQC Report
Mon 6 Jun 2016
ERR1390704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1390704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575091
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA651.4040779E-831.14662219
AGATCGG602.420329E-729.99304417
TCGGAAG702.8898285E-828.91934820
GATCGGA654.8761285E-727.68588618
GAGCGGT903.2911157E-722.49282626
GCGGTTC955.534148E-721.31084828
CGGAAGA855.0388917E-621.1697221
CGGTTCA1109.9867975E-820.44980229
GCGGAGC550.0039394720.44980232
AGAGCGG1009.06648E-720.24354425
TCGTTGG1251.7324055E-819.8298954
CCTTCGT951.2969756E-518.9759791
CGTTGGA1202.4475412E-718.781585
CCGAGAT1202.497345E-718.74565114
ACCCGGC600.006515349318.74565114
TGAGCCG851.2060575E-418.52350445
CTTCGTT1002.0173833E-518.0271782
TTCGTTG1253.7648715E-718.0271783
AGCGGTT1153.4502227E-617.60461227
CCGAGAC1153.4530876E-617.60308345
Produced by FastQC (version 0.11.5)